Nidogen antibody (AA 971-1219)
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- Target See all Nidogen (NID) products
- Nidogen (NID)
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Binding Specificity
- AA 971-1219
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Nidogen antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Nidogen (NID)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against NID. It has been selected for its ability to recognize NID in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Mouse, Rat
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Nidogen (NID) corresdonding to Ala971~Cys1219 with N-terminal His Tag
- Isotype
- IgG
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- Application Notes
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Western blotting: 0.5-2 μg/mL
Immunohistochemistry: 5-20 μg/mL
Immunocytochemistry: 5-20 μg/mL
Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- Nidogen (NID)
- Alternative Name
- Nidogen (NID Products)
- Synonyms
- BEST:CK00552 antibody, CG12908 antibody, CT32053 antibody, Dmel\\CG12908 antibody, ndg antibody, Nidogen/entactin antibody, Ndg antibody
- Background
- Entactin
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