MED1 antibody (AA 1-212)

Catalog No. ABIN7444733

Product Details anti-MED1 Antibody

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Binding Specificity: AA 1-212

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Mediator Complex Subunit 1 (MED1)

Specificity: The antibody is a rabbit polyclonal antibody raised against MED1. It has been selected for its ability to recognize MED1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Mediator Complex Subunit 1 (MED1) corresdonding to Met1~Thr212 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for MED1

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Alternative Name: Mediator Complex Subunit 1 (MED1 Products)

Background: PPARBP, ARC205, PBP, CRSP1, CRSP200, PPARGBP, RB18A, TRAP220, TRIP2, Peroxisome proliferator-activated receptor-binding protein, Thyroid receptor-interacting protein 2

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha