SMAD5 antibody (AA 210-464)

Catalog No. ABIN7441760

Product Details anti-SMAD5 Antibody

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Binding Specificity: AA 210-464

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Purpose: Polyclonal Antibody to SMAD family member 5 (SMAD5)

Specificity: The antibody is a rabbit polyclonal antibody raised against SMAD5. It has been selected for its ability to recognize SMAD5 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant SMAD family member 5 (SMAD5) corresdonding to Gly210~Val464 (Accession # Q99717)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:500-2000 Immunohistochemistry: 5-20 μg/mL

1:50-200 Immunocytochemistry: 5-20 μg/mL

1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SMAD5

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Alternative Name: SMAD family member 5 (SMAD5 Products)

Synonyms: DWFC antibody, JV5-1 antibody, MADH5 antibody, Madh5 antibody, SBN antibody, fb67b04 antibody, mad antibody, madh5 antibody, sb:cb400 antibody, smd5 antibody, wu:fb67b04 antibody, SMAD5 antibody, DKFZp468M0619 antibody, 1110051M15Rik antibody, AI451355 antibody, Dwf-C antibody, MusMLP antibody, SMAD family member 5 antibody, SMAD5 antibody, Smad5 antibody, smad5 antibody

Background: MADH5, Dwfc, JV5-1, Mothers Against Decapentaplegic Homolog 5