Choline Acetyltransferase antibody (AA 517-732)

Catalog No. ABIN7440704

Product Details anti-Choline Acetyltransferase Antibody

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Binding Specificity: AA 517-732

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Choline Acetyltransferase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Polyclonal Antibody to Choline Acetyltransferase (ChAT)

Specificity: The antibody is a rabbit polyclonal antibody raised against ChAT. It has been selected for its ability to recognize ChAT in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Choline Acetyltransferase (ChAT) corresdonding to Asp517~Pro732 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Alternative Name: Choline Acetyltransferase (CHAT Products)

Synonyms: CHOACTASE antibody, CMS1A antibody, CMS1A2 antibody, ChoAcTase antibody, B230380D24Rik antibody, choline O-acetyltransferase antibody, choline O-acetyltransferase a antibody, choline acetyltransferase antibody, carnitine O-acetyltransferase antibody, CHAT antibody, chata antibody, Chat antibody, PGTG_00551 antibody, LOC100533322 antibody

Background: CMS1A, CMS1A2, CHOACTase, Choline acetylase

Pathways: Skeletal Muscle Fiber Development