Aggrecan antibody (AA 34-147)

Catalog No. ABIN7437053

Product Details anti-Aggrecan Antibody

Target: Aggrecan (ACAN)

Binding Specificity: AA 34-147

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aggrecan antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Aggrecan (AGC)

Specificity: The antibody is a rabbit polyclonal antibody raised against AGC. It has been selected for its ability to recognize AGC in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Aggrecan (AGC) corresdonding to Pro34~Glu147 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Aggrecan

Target: Aggrecan (ACAN)

Alternative Name: Aggrecan (ACAN Products)

Synonyms: AGC1 antibody, AGCAN antibody, CSPG1 antibody, CSPGCP antibody, MSK16 antibody, SEDK antibody, Agc antibody, Agc1 antibody, Cspg1 antibody, b2b183Clo antibody, cmd antibody, aggrecan antibody, aggrecan core protein antibody, ACAN antibody, Acan antibody, LOC100380783 antibody, AGC antibody

Background: ACAN, AGC1, AGCAN, CSPG1, CSPCP, CSPGCP, MSK16, SEDK, Cartilage-specific proteoglycan core protein, Large Aggregating Proteoglycan, Chondroitin Sulfate Proteoglycan 1

Pathways: Glycosaminoglycan Metabolic Process, Dicarboxylic Acid Transport