NFkB antibody (AA 805-892)

Catalog No. ABIN7442525

Product Details anti-NFkB Antibody

Key Features:

• High quality NFkB Primary Antibody for the detection of NFkB.
• Reliable product with high standard validation data

Target: NFkB (Nuclear Factor kappa B (NFkB))

Binding Specificity: AA 805-892

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFkB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Nuclear Factor Kappa B (NFkB)

Specificity: The antibody is a rabbit polyclonal antibody raised against NFkB. It has been selected for its ability to recognize NFkB in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Nuclear Factor Kappa B (NFkB) corresdonding to Gln805~Ser892 (Accession # P19838) with N-terminal His and GST Tag

Isotype: IgG

Product Specific Information:

What can the NFkB Antibody ABIN7442525 be used for?: This polyclonal NFkB Antibody detects NFkB. The NFkB Antibody has been validated for various applications and can be used for the detection of NFkB and derivatives by Western Blotting, Immunohistochemistry, Immunoprecipitation, Immunocytochemistry.

What validation data is available for this NFkB Antibody?: The primary antibody has currently 6 product images that show its performance in a variety of applications. The product is currently available in 1 mL, 20 μL, 100 μL, 200 μL quantities. NFkB Antibody for the detection of NFkB and derivatives.

What is the function of NFkB?: This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009].

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for NFkB

Target: NFkB (Nuclear Factor kappa B (NFkB))

Alternative Name: Nuclear Factor Kappa B (NFkB Products)

Synonyms: EBP-1 antibody, KBF1 antibody, NF-kB1 antibody, NF-kappa-B antibody, NF-kappaB antibody, NFKB-p105 antibody, NFKB-p50 antibody, NFkappaB antibody, p105 antibody, p50 antibody, NF-kB antibody, NF-KB1 antibody, NF-kappaB1 antibody, p50/p105 antibody, nuclear factor kappa B subunit 1 antibody, nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 antibody, NFKB1 antibody, Nfkb1 antibody

Background: NF-KB1, EBP-1, KBF1, NF-Kappa-B, NFKB-P105, NFKB-P50, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1, Nuclear factor NF-kappa-B p105 subunit

Pathways: Ubiquitin Proteasome Pathway, S100 Proteins