Pituitary Adenylate Cyclase Activating Peptide (AA 17-176) antibody
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- Target
- Pituitary Adenylate Cyclase Activating Peptide
- Binding Specificity
- AA 17-176
- Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
- Application
- Immunocytochemistry (ICC), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Polyclonal Antibody to Pituitary Adenylate Cyclase Activating Peptide (PACAP)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against PACAP. It has been selected for its ability to recognize PACAP in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Mouse
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Pituitary Adenylate Cyclase Activating Peptide (PACAP) corresdonding to Ile17~Leu176 with N-terminal His and GST Tag
- Isotype
- IgG
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- Application Notes
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Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- Pituitary Adenylate Cyclase Activating Peptide
- Synonyms
- PACAP antibody, adenylate cyclase activating polypeptide 1 antibody, ADCYAP1 antibody, Adcyap1 antibody
- Background
- ADCYAP1, PRP-48, Pituitary Adenylate Cyclase Activating Peptide
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