Perforin 1 antibody (AA 32-316)

Catalog No. ABIN7433450

Product Details anti-Perforin 1 Antibody

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Binding Specificity: AA 32-316

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Perforin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Perforin 1 (PRF1)

Specificity: The antibody is a rabbit polyclonal antibody raised against PRF1. It has been selected for its ability to recognize PRF1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Perforin 1 (PRF1) corresdonding to Lys32~Phe316 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Alternative Name: Perforin 1 (PRF1 Products)

Synonyms: FLH2 antibody, HPLH2 antibody, P1 antibody, PFN1 antibody, PFP antibody, PRF1 antibody, Pfn antibody, Pfp antibody, Prf-1 antibody, Cyta antibody, RATCYTA antibody, LOC443187 antibody, perforin antibody, prf1 antibody, cytolysin antibody, perforin-1 antibody, perforin-1-like antibody, perforin 1 antibody, perforin 1 (pore forming protein) antibody, perforin antibody, perforin 1 L homeolog antibody, PRF1 antibody, Prf1 antibody, LOC443187 antibody, prf1 antibody, prf1.L antibody

Background: FLH2, HPLH2, P1, PFP, Pore Forming Protein, Cytolysin, Lymphocyte pore-forming protein

Pathways: Apoptosis, Caspase Cascade in Apoptosis