Nephrin antibody (AA 27-234)
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- Target See all Nephrin (NPHS1) Antibodies
- Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))
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Binding Specificity
- AA 27-234
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Nephrin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Nephrin (NPHN)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against NPHN. It has been selected for its ability to recognize NPHN in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Mouse, Rat
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Nephrin (NPHN) corresdonding to Pro27~Val234 with N-terminal His Tag
- Isotype
- IgG
- Top Product
- Discover our top product NPHS1 Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
1:500-2000 Immunohistochemistry: 5-20 μg/mL
1:50-200 Immunocytochemistry: 5-20 μg/mL
1:50-200 Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))
- Alternative Name
- Nephrin (NPHS1 Products)
- Synonyms
- NPHS1 antibody, nphs1l antibody, nphs1 antibody, CNF antibody, NPHN antibody, nephrin antibody, NephrinB antibody, NPHS1, nephrin antibody, nephrosis 1, congenital, Finnish type (nephrin) antibody, NPHS1 nephrin S homeolog antibody, nephrin antibody, NPHS1 nephrin antibody, nephrosis 1, nephrin antibody, NPHS1 antibody, nphs1 antibody, nphs1.S antibody, LOC5579127 antibody, Nphs1 antibody
- Background
- NPHS1, CNF, Nephrosis 1, Congenital Finnish Type, Renal glomerulus-specific cell adhesion receptor
- Pathways
- Regulation of Actin Filament Polymerization, Skeletal Muscle Fiber Development
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