CRH antibody (AA 40-195)

Catalog No. ABIN7429697

Product Details anti-CRH Antibody

Target: CRH (Corticotropin Releasing Hormone (CRH))

Binding Specificity: AA 40-195

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Corticotropin Releasing Factor (CRF)

Specificity: The antibody is a rabbit polyclonal antibody raised against CRF. It has been selected for its ability to recognize CRF in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Corticotropin Releasing Factor (CRF) corresdonding to His40~Gly195 (Accession # P06850) with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:500-2000 Immunohistochemistry: 5-20 μg/mL

1:50-200 Immunocytochemistry: 5-20 μg/mL

1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CRH

Target: CRH (Corticotropin Releasing Hormone (CRH))

Alternative Name: Corticotropin Releasing Factor (CRH Products)

Synonyms: CRF antibody, Gm1347 antibody, crf antibody, crh antibody, zgc:101864 antibody, CRH antibody, corticoliberin antibody, crhb antibody, crh.1-a antibody, crha antibody, corticotropin releasing hormone antibody, corticotropin releasing hormone b antibody, corticotropin releasing hormone S homeolog antibody, corticotropin releasing hormone L homeolog antibody, Crh antibody, CRH antibody, crhb antibody, crh antibody, crh.S antibody, crh.L antibody

Background: CRH, Corticotropin Releasing Hormone, Corticoliberin

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Feeding Behaviour