HSP27 antibody (AA 1-201)

Catalog No. ABIN7432406

Product Details anti-HSP27 Antibody

Target: HSP27 (Heat Shock Protein 27 (HSP27))

Binding Specificity: AA 1-201

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Purpose: Polyclonal Antibody to Heat Shock Protein 27 (Hsp27)

Specificity: The antibody is a rabbit polyclonal antibody raised against Hsp27. It has been selected for its ability to recognize Hsp27 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Heat Shock Protein 27 (Hsp27) corresdonding to Met1~Lys201 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:500-2000 Immunohistochemistry: 5-20 μg/mL

1:50-200 Immunocytochemistry: 5-20 μg/mL

1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for HSP27

Target: HSP27 (Heat Shock Protein 27 (HSP27))

Alternative Name: Heat Shock Protein 27 (HSP27 Products)

Synonyms: 27 antibody, 27K antibody, 28 antibody, CG4466 antibody, Dhsp27 antibody, DmHSP27 antibody, DmHsp27 antibody, Dmel\\CG4466 antibody, HSP27 antibody, HSP28 antibody, Hsp 27 antibody, Hsp28 antibody, anon-WO0140519.69 antibody, hsp 27 antibody, hsp-27 antibody, hsp26 antibody, hsp27 antibody, hsp27/26 antibody, hsp28 antibody, small hsp locus 67B antibody, Heat shock protein 27 antibody, heat shock protein 27 antibody, Hsp27 antibody, CpipJ_CPIJ005642 antibody

Background: HSPB1, HSP-B1, CMT2F, HSP28, Hsp25, Heat Shock 27 kDa Protein 1, 28 kDa heat shock protein, Heat shock protein beta-1, Estrogen-regulated 24 kDa protein,

Pathways: VEGF Signaling