Hydroxyproline antibody
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- Target See all Hydroxyproline (Hyp) Antibodies
- Hydroxyproline (Hyp)
- Reactivity
- Various Species
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Hydroxyproline antibody is un-conjugated
- Application
- Chemiluminescence Immunoassay (CLIA), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Purpose
- Polyclonal Antibody to Hydroxyproline (Hyp)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against Hyp. It has been selected for its ability to recognize Hyp in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Various Species
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- BSA Conjugated Hydroxyproline (Hyp)
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry: 5-20 μg/mL Immunocytochemistry: 5-20 μg/mL Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- Hydroxyproline (Hyp)
- Alternative Name
- Hydroxyproline (Hyp Products)
- Target Type
- Amino Acid
- Background
- (2S,4R)-4-Hydroxyproline, L-Hydroxyproline(C5H9O3N)
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