PCNA antibody (AA 6-261)

Catalog No. ABIN7443113

Product Details anti-PCNA Antibody

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Binding Specificity: AA 6-261

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Dog, Rhesus Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)

Specificity: The antibody is a rabbit polyclonal antibody raised against PCNA. It has been selected for its ability to recognize PCNA in immunohistochemical staining and western blotting.

Cross-Reactivity: Chicken, Cow, Dog, Goat, Guinea Pig, Horse, Mouse, Pig, Rat, Sheep

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Proliferating Cell Nuclear Antigen (PCNA) corresdonding to Leu6~Ser261 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-3 μg/mL

Immunohistochemistry: 5-30 μg/mL

Immunocytochemistry: 5-30 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: Proliferating Cell Nuclear Antigen (PCNA Products)

Background: Cyclin

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy