PKN1 antibody (AA 615-874)

Catalog No. ABIN7439912

Product Details anti-PKN1 Antibody

Target: PKN1 (Protein Kinase N1 (PKN1))

Binding Specificity: AA 615-874

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Polyclonal Antibody to Protein Kinase N1 (PKN1)

Specificity: The antibody is a rabbit polyclonal antibody raised against PKN1. It has been selected for its ability to recognize PKN1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Protein Kinase N1 (PKN1) corresdonding to Phe615~Phe874 (Accession # Q16512)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:500-2000 Immunohistochemistry: 5-20 μg/mL

1:50-200 Immunocytochemistry: 5-20 μg/mL

1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PKN1

Target: PKN1 (Protein Kinase N1 (PKN1))

Alternative Name: Protein Kinase N1 (PKN1 Products)

Background: PAK1, DBK, PRK1, PRKCL1, Protein Kinase C-Like 1, Protein kinase PKN-alpha, Protein-kinase C-related kinase 1, Serine-threonine protein kinase N