Endothelin 1 antibody (AA 53-90)

Catalog No. ABIN7429727

Product Details anti-Endothelin 1 Antibody

Target: Endothelin 1 (EDN1)

Binding Specificity: AA 53-90

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Endothelin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Endothelin 1 (EDN1)

Specificity: The antibody is a rabbit polyclonal antibody raised against EDN1. It has been selected for its ability to recognize EDN1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Endothelin 1 (EDN1) corresdonding to Cys53~Ser90 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Endothelin 1

Target: Endothelin 1 (EDN1)

Alternative Name: Endothelin 1 (EDN1 Products)

Synonyms: et1 antibody, et-1 antibody, Edn1 antibody, EDN antibody, ET-1 antibody, ET1 antibody, PPET1 antibody, HDLCQ7 antibody, preproET antibody, Et1 antibody, fb14d01 antibody, wu:fb14d01 antibody, PPET-1 antibody, EDN1 antibody, edn1 antibody, edn1-a antibody, edn1-b antibody, endothelin 1 antibody, endothelin 1 S homeolog antibody, endothelin 1 L homeolog antibody, edn1 antibody, EDN1 antibody, Edn1 antibody, edn1.S antibody, edn1.L antibody

Background: ET1, PPET1, Preproendothelin-1, Big endothelin-1

Pathways: Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size