PNPLA2 antibody (AA 484-701)

Catalog No. ABIN7431758

Product Details anti-PNPLA2 Antibody

Target: PNPLA2 (Calcium Independent Phospholipase A2 (PNPLA2))

Binding Specificity: AA 484-701

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PNPLA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Polyclonal Antibody to Phospholipase A2, Calcium Independent (iPLA2)

Specificity: The antibody is a rabbit polyclonal antibody raised against iPLA2. It has been selected for its ability to recognize iPLA2 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Phospholipase A2, Calcium Independent (iPLA2) corresdonding to Arg484~Ile701 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PNPLA2

Target: PNPLA2 (Calcium Independent Phospholipase A2 (PNPLA2))

Alternative Name: Phospholipase A2, Calcium Independent (PNPLA2 Products)

Synonyms: 1110001C14Rik antibody, ATGL antibody, PEDF-R antibody, TTS-2.2 antibody, TTS2 antibody, iPLA2zeta antibody, BB112799 antibody, PNPLA9 antibody, iPLA(2)beta antibody, iPLA2 antibody, iPLA2beta antibody, patatin like phospholipase domain containing 2 antibody, phospholipase A2, group VI antibody, PNPLA2 antibody, Pla2g6 antibody

Background: PLA2G6, CaI-PLA2, GVI, INAD1, PNPLA9, Ca2+ Independent PLA2, 85 kDa Calcium-Independent Phospholipase A2, Phospholipase A2,group VI(Cytosolic,Calcium-Independent)