Orexin antibody (AA 34-97)

Catalog No. ABIN7440371

Product Details anti-Orexin Antibody

Target: Orexin (OX)

Binding Specificity: AA 34-97

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Orexin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Orexin (OX)

Specificity: The antibody is a rabbit polyclonal antibody raised against OX. It has been selected for its ability to recognize OX in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Orexin (OX) corresdonding to Gln34~Met97 with Two N-terminal Tags, His-tag and SUMO-tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.2-2 μg/mL

1:250-2500 Immunohistochemistry: 5-20 μg/mL

1:25-100 Immunocytochemistry: 5-20 μg/mL

1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Orexin

Target: Orexin (OX)

Alternative Name: Orexin (OX Products)

Synonyms: orexin-A antibody, PPOX antibody, NRCLP1 antibody, OX antibody, hypocretin neuropeptide precursor antibody, hypocretin antibody, Hcrt antibody, HCRT antibody

Background: HCRT, PPOX, Orexin A, Hypocretin 1, Orexin B, Hypocretin 2, Preproorexin, Hypocretin Neuropeptide Precursor

Pathways: Synaptic Membrane, Feeding Behaviour