Ubiquitin antibody (AA 1-229)

Catalog No. ABIN7443803

Product Details anti-Ubiquitin Antibody

Target: Ubiquitin

Binding Specificity: AA 1-229

Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Purpose: Polyclonal Antibody to Ubiquitin (Ub)

Specificity: The antibody is a rabbit polyclonal antibody raised against Ub. It has been selected for its ability to recognize Ub in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rabbit, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Ubiquitin (Ub) corresdonding to Met1~Cys229 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL Immunohistochemistry: 5-20 μg/mL Immunocytochemistry: 5-20 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Ubiquitin

Target: Ubiquitin

Alternative Name: Ubiquitin (Ubiquitin Products)

Background: Ubiquitin B, UbB, Ubiquitous Immunopoietic Polypeptide, Polyubiquitin B

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway