TEK antibody (AA 286-465)

Catalog No. ABIN7442285

Product Details anti-TEK Antibody

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Binding Specificity: AA 286-465

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TEK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to TEK Tyrosine Kinase, Endothelial (Tie2)

Specificity: The antibody is a rabbit polyclonal antibody raised against Tie2. It has been selected for its ability to recognize Tie2 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant TEK Tyrosine Kinase, Endothelial (Tie2) corresdonding to Gly286~Ile465 (Accession # Q02763)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for TEK

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Alternative Name: TEK Tyrosine Kinase, Endothelial (TEK Products)

Synonyms: TEK antibody, DKFZp469E064 antibody, AA517024 antibody, Cd202b antibody, Hyk antibody, Tie2 antibody, tie-2 antibody, CD202B antibody, TIE-2 antibody, TIE2 antibody, VMCM antibody, VMCM1 antibody, Tie-2 antibody, tie2 antibody, TEK receptor tyrosine kinase antibody, angiopoietin-1 receptor antibody, TEK tyrosine kinase, endothelial antibody, TEK antibody, LOC100408334 antibody, tek antibody, Tek antibody

Background: CD202b, CD202-B, TEK, VMCM1, Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains 2, Venous Malformations Multiple Cutaneous And Mucosal

Pathways: RTK Signaling, Growth Factor Binding