LIF antibody (AA 25-203)

Catalog No. ABIN7430885

Product Details anti-LIF Antibody

Target: LIF (Leukemia Inhibitory Factor (LIF))

Binding Specificity: AA 25-203

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Leukemia Inhibitory Factor (LIF)

Specificity: The antibody is a rabbit polyclonal antibody raised against LIF. It has been selected for its ability to recognize LIF in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Leukemia Inhibitory Factor (LIF) corresdonding to Pro25~Phe203 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:200-800 Immunohistochemistry: 5-20 μg/mL

1:20-80 Immunocytochemistry: 5-20 μg/mL

1:20-80 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for LIF

Target: LIF (Leukemia Inhibitory Factor (LIF))

Alternative Name: Leukemia Inhibitory Factor (LIF Products)

Synonyms: CDF antibody, DIA antibody, HILDA antibody, MLPLI antibody, LIF, interleukin 6 family cytokine antibody, leukemia inhibitory factor antibody, LIF antibody, Lif antibody

Background: CDF, D-FACTOR, HILDA, MLPLI, Cholinergic Differentiation Factor, Differentiation-Stimulating Factor, Melanoma-Derived LPL Inhibitor, Emfilermin

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding