ECF antibody (AA 24-97)

Catalog No. ABIN7430438

Product Details anti-ECF Antibody

Target: ECF (Eosinophil Chemotactic Factor (ECF))

Binding Specificity: AA 24-97

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ECF antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Eosinophil Chemotactic Factor (ECF)

Specificity: The antibody is a rabbit polyclonal antibody raised against ECF. It has been selected for its ability to recognize ECF in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Eosinophil Chemotactic Factor (ECF) corresdonding to His24~Pro97 (Accession # P97545) with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for ECF

Target: ECF (Eosinophil Chemotactic Factor (ECF))

Alternative Name: Eosinophil Chemotactic Factor (ECF Products)

Synonyms: Scya11 antibody, C-C motif chemokine ligand 11 antibody, Ccl11 antibody

Background: CCL11, SCYA11, Eotaxin 1, Eosinophil Chemotactic Protein 1, Chemokine C-C-Motif Ligand 11, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 11