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Sonic Hedgehog antibody (AA 36-193)

SHH Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal D6 unconjugated
Catalog No. ABIN7426367
  • Target See all Sonic Hedgehog (SHH) Antibodies
    Sonic Hedgehog (SHH)
    Binding Specificity
    • 15
    • 12
    • 9
    • 6
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 36-193
    Reactivity
    • 66
    • 50
    • 37
    • 17
    • 10
    • 6
    • 6
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 74
    • 11
    • 8
    • 1
    • 1
    Mouse
    Clonality
    • 68
    • 27
    Monoclonal
    Conjugate
    • 52
    • 8
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Sonic Hedgehog antibody is un-conjugated
    Application
    • 69
    • 28
    • 22
    • 14
    • 13
    • 11
    • 10
    • 7
    • 6
    • 5
    • 5
    • 4
    • 3
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Purpose
    Monoclonal Antibody to Hedgehog Homolog, Sonic (SHH)
    Specificity
    The antibody is a mouse monoclonal antibody raised against SHH. It has been selected for its ability to recognize SHH in immunohistochemical staining and western blotting.
    Purification
    Protein A + Protein G affinity chromatography
    Immunogen
    Recombinant Hedgehog Homolog, Sonic (SHH) corresdonding to Pro36~Ala193 with N-terminal His Tag
    Clone
    D6
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product SHH Primary Antibody
  • Application Notes

    Western blotting: 0.5-2 μg/mL

    1:500-2000 Immunohistochemistry: 5-20 μg/mL

    1:50-200 Immunocytochemistry: 5-20 μg/mL

    1:50-200 Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Expiry Date
    24 months
  • Target
    Sonic Hedgehog (SHH)
    Alternative Name
    Hedgehog Homolog, Sonic (SHH Products)
    Background
    HHG1, HLP3, HPE3, SMMCI, SHHN, SHH-N
    Pathways
    Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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