MTOR antibody (AA 2226-2488)

Catalog No. ABIN7427519

Product Details anti-MTOR Antibody

Target: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Binding Specificity: AA 2226-2488

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MTOR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to Serine/threonine-protein kinase mTOR (mTOR)

Specificity: The antibody is a mouse monoclonal antibody raised against mTOR. It has been selected for its ability to recognize mTOR in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Serine/threonine-protein kinase mTOR (mTOR) corresdonding to Ala2226~Val2488 with N-terminal His Tag

Clone: D4

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-5 μg/mL Immunohistochemistry: 5-30 μg/mL Immunocytochemistry: 5-30 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for MTOR

Target: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Alternative Name: Serine/threonine-protein kinase mTOR (mTOR Products)

Background: FRAP, FRAP1, FRAP2, MTOR, RAFT1, RAPT1, FK506 Binding Protein 12 Rapamycin Associated Protein, Mammalian Target Of Rapamycin, FKBP12-Rapamycin Complex-Associated Protein 1, Rapamycin and FKBP12 target

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effect