EGFR antibody (AA 888-1210)

Catalog No. ABIN7426025

Product Details anti-EGFR Antibody

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Binding Specificity: AA 888-1210

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to Epidermal Growth Factor Receptor (EGFR)

Specificity: The antibody is a mouse monoclonal antibody raised against EGFR. It has been selected for its ability to recognize EGFR in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Epidermal Growth Factor Receptor (EGFR) corresdonding to His888~Ala1210 with N-terminal His Tag

Clone: C4

Isotype: IgG2b kappa

Application Details

Application Notes:

Western blotting: 0.5-5 μg/mL

Immunohistochemistry: 5-50 μg/mL

Immunocytochemistry: 5-50 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: Epidermal Growth Factor Receptor (EGFR Products)

Background: ErbB-1, ErbB1, ER1, ERB-B1, MENA, HER1, Erythroblastic Leukemia Viral(v-Erb-B)oncogene Homolog,Avian, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins