Amphiregulin antibody (AA 20-100)

Catalog No. ABIN7428263

Product Details anti-Amphiregulin Antibody

Target: Amphiregulin (AREG)

Binding Specificity: AA 20-100

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Amphiregulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to Amphiregulin (AREG)

Specificity: The antibody is a mouse monoclonal antibody raised against AREG. It has been selected for its ability to recognize AREG in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Amphiregulin (AREG) corresdonding to Ser20~Asp100 with N-terminal His and GST Tag

Clone: A5

Isotype: IgG2b kappa

Application Details

Application Notes:

Western blotting: 0.5-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-30 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-30 μg/mL Immunohistochemistry in paraffin section: 5-30 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Amphiregulin

Target: Amphiregulin (AREG)

Alternative Name: Amphiregulin (AREG Products)

Synonyms: AR antibody, CRDGF antibody, SDGF antibody, Sdgf antibody, AREG antibody, AREGB antibody, AREG/SDGF antibody, amphiregulin antibody, AREG antibody, Areg antibody

Background: AR, CRDGF, SDGF, Colorectum Cell-Derived Growth Factor, Schwannoma-Derived Growth Factor

Pathways: RTK Signaling, EGFR Signaling Pathway