HOOK3 antibody (N-Term)

Catalog No. ABIN6992177

Product Details anti-HOOK3 Antibody

Target: HOOK3 (Hook Homolog 3 (HOOK3))

Binding Specificity: AA 210-260, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HOOK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: HOOK3 antibody is human, mouse and rat reactive. HOOK3 antibody is predicted to not cross-react with HOOK1 and HOOK2.

Purification: HOOK3 Antibody is affinity chromatography purified via peptide column.

Immunogen: HOOK3 antibody was raised against a 19 amino acid peptide from near the amino terminus of human HOOK3. The immunogen is located within amino acids 210 - 260 of HOOK3.

Isotype: IgG

Application Details

Application Notes: HOOK3 antibody can be used for the detection of HOOK3 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry at 10 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: HOOK3 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: HOOK3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for HOOK3

Target: HOOK3 (Hook Homolog 3 (HOOK3))

Alternative Name: HOOK3 (HOOK3 Products)

Synonyms: 5830454D03Rik antibody, AI317159 antibody, E330005F07Rik antibody, HK3 antibody, hook3 antibody, hook microtubule-tethering protein 3 antibody, hook microtubule tethering protein 3 antibody, hook microtubule-tethering protein 3 L homeolog antibody, Hook3 antibody, HOOK3 antibody, hook3.L antibody

Background: The HOOK proteins (HOOK1, HOOK2 and HOOK3) comprise a family of cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules mediating the spatial organization of diverse membrane-trafficking systems, their more divergent C-terminal domains mediate binding to organelles (1,2). HOOK3 exists as a homodimer and participates in defining the architecture and localization of the Golgi complex through its central coiled-coil domain (3). HOOK3 may regulate clearance of endocytosed receptors such as MSR1 (3,4).

Molecular Weight: Predicted: 79 kDa

Observed: 77 kDa

Gene ID: 84376

NCBI Accession: NP_115786

UniProt: Q86VS8

Pathways: Stem Cell Maintenance, Maintenance of Protein Location