ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Specificity
UBAP2L antibody is human and mouse reactive. At least four isoforms of UBAP2L are known to exist, this antibody will detect all four isoforms. This antibody is predicted to not cross-react with UBAP2.
Purification
UBAP2L Antibody is affinity chromatography purified via peptide column.
Immunogen
UBAP2L antibody was raised against a 19 amino acid peptide from near the amino terminus of human UBAP2L. The immunogen is located within amino acids 200 - 250 of UBAP2L.
UBAP2L
Reactivity: Human
WB, IP
Host: Rabbit
Polyclonal
unconjugated
Application Notes
UBAP2L antibody can be used for the detection of UBAP2L by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry at 10 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
UBAP2L Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
UBAP2L antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Target
UBAP2L
(Ubiquitin Associated Protein 2-Like (UBAP2L))
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Background
The UBA domain-containing protein ubiquitin associated protein 2-like (UBAP2L), also known as NICE4, is a ubiquitously expressed 1,087 amino acid protein. UBAP2L is preferentially expressed in hematopoietic stem cell (HSC) populations and interacts with BMI1, a well-known determinant of HSC function (1). It is essential for the activity of long-term repopulating HSCs but not the BMI1-dependent repression of the Ink4a/Arf locus (1).