Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
VTI1b antibody is human, mouse and rat reactive. At least three isoforms of VTI1a are known to exist, this antibody will detect all three isoforms. VTI1b antibody is predicted to not cross-react with VTI1a.
Purification
VTI1b antibody is affinity chromatography purified via peptide column.
Immunogen
VTI1b antibody was raised against a 17 amino acid peptide near the center of human VTI1b. The immunogen is located within amino acids 150 - 200 of VTI1b.
VTI1b antibody can be used for detection of VTI1b by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
VTI1b antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
VTI1b antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Target
VTI1B
(Vesicle Transport through Interaction with T-SNAREs Homolog 1B (VTI1B))
VTI1 antibody, VTI1-LIKE antibody, VTI1L antibody, VTI2 antibody, v-SNARE antibody, vti1-rp1 antibody, AU015348 antibody, GES30 antibody, MVti1b antibody, SNARE antibody, Vti1-rp1 antibody, zgc:63525 antibody, VTI1B antibody, vesicle transport through interaction with t-SNAREs 1B antibody, vesicle transport through interaction with t-SNAREs 1B pseudogene antibody, VTI1B antibody, Vti1b antibody, vti1b antibody, LOC461624 antibody
Background
Vesicle transport through interaction with t-SNAREs homolog 1 (VTI1a and VTI1b) are involved in vesicular transport from the late endosomes to the trans-Golgi network (1). They are both localized in the trans-Golgi network, with VTI1a also found in the Golgi apparatus and VTI1b in endosomes (2,3). It is thought that VTI1b along with the SNARE protein VAMP8 mediates the fusion of antimicrobial and canonical autophagosomes with lysosomes, an essential process for autophagy (4).