IZUMO1 antibody (AA 170-220)

Catalog No. ABIN6992154

Product Details anti-IZUMO1 Antibody

Target: IZUMO1 (Izumo Sperm-Egg Fusion 1 (IZUMO1))

Binding Specificity: AA 170-220

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IZUMO1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: IZUMO1 antibody is human specific. At least four isoforms of IZUMO1 are known to exist, this antibody will detect the two longest isoforms.

Purification: IZUMO1 antibody is affinity chromatography purified via peptide column.

Immunogen: IZUMO1 antibody was raised against an 18 amino acid peptide near the center of human IZUMO1. The immunogen is located within amino acids 170 - 220 of IZUMO1.

Isotype: IgG

Application Details

Application Notes: IZUMO1 antibody can be used for detection of IZUMO1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: IZUMO1 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: IZUMO1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for IZUMO1

Target: IZUMO1 (Izumo Sperm-Egg Fusion 1 (IZUMO1))

Alternative Name: IZUMO1 (IZUMO1 Products)

Synonyms: IZUMO antibody, 1700058F15Rik antibody, Izumo antibody, izumo sperm-egg fusion 1 antibody, Ras interacting protein 1 antibody, IZUMO1 antibody, RASIP1 antibody, Izumo1 antibody

Background: IZUMO1, a sperm-specific protein, is essential for sperm-egg plasma membrane binding and fusion. It is involved in the fertilization process and in the fusion of the sperm with the egg (1,2). IZUMO1 is expressed as at least four isoforms produced by alternative splicing and contains one Ig-like (immunoglobulin-like) C2-type domain (3). Lack of IZUMO1 in males, due to homozygous mutation in the gene that encodes IZUMO1, results in sterility due to an inability to penetrate the plasma membrane of the egg (4).

Molecular Weight: Predicted: 35, 39, 42 kDa

Observed: 52 kDa

Gene ID: 284359

NCBI Accession: NP_872381

UniProt: Q8IYV9