Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
Specificity
SLCO1B1 antibody is human specific. At least four isoforms of SLCO1B1 are known to exist. This antibody is predicted to not cross-react with other SLCO1B proteins.
Purification
SLCO1B1 antibody is affinity chromatography purified via peptide column.
Immunogen
SLCO1B1 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human SLCO1B1. The immunogen is located within the last 50 amino acids of SLCO1B1.
Verified
SLCO1B1
Reactivity: Human
ELISA, IF
Host: Goat
Polyclonal
unconjugated
Application Notes
SLCO1B1 antibody can be used for detection of SLCO1B1 by Western blot at 1 - 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
SLCO1B1 antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
SLCO1B1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Target
SLCO1B1
(Solute Carrier Organic Anion Transporter Family, Member 1B1 (SLCO1B1))
SLCO1B1 is a transmembrane receptor that mediates the sodium-independent uptake of numerous endogenous compounds including bilirubin, 17-beta-glucuronosyl estradiol and may play an important role in the clearance of bile acids and organic anions from the liver (1,2). It contains one Kazal-like domain and belongs to the organo-anion transporter family (2,3). SLCO1B1 is highly expressed in liver and is localized to the basolateral hepatocyte membrane. It is responsible for the hepatic uptake of the liver-specific hydroxymethylglutaryl-CoA reductase inhibitor in mouse, rat and human (3,4).