DIRAS3 antibody (C-Term)

Catalog No. ABIN6992120

Product Details anti-DIRAS3 Antibody

Target: DIRAS3 (DIRAS Family, GTP-Binding RAS-Like 3 (DIRAS3))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIRAS3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: DIRAS3 antibody is human specific.

Purification: DIRAS3 antibody is affinity chromatography purified via peptide column.

Immunogen: DIRAS3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DIRAS3. The immunogen is located within the last 50 amino acids of DIRAS3.

Isotype: IgG

Application Details

Application Notes: DIRAS3 antibody can be used for detection of DIRAS3 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: DIRAS3 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: DIRAS3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for DIRAS3

Target: DIRAS3 (DIRAS Family, GTP-Binding RAS-Like 3 (DIRAS3))

Alternative Name: DIRAS3 (DIRAS3 Products)

Synonyms: DIRAS3 antibody, arhi antibody, noey2 antibody, ARHI antibody, NOEY2 antibody, DIRAS family GTPase 3 antibody, DIRAS family GTP binding RAS like 3 antibody, DIRAS3 antibody, diras3 antibody

Background: DIRAS3 is a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with mono-allelic expression of the paternal allele, which is associated with growth suppression and down-regulation of cyclin D1 promoter activity and induction of p21 (WAF/CIP1). Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers (1). DIRAS3 has been shown to induce autophagy in human ovarian cancer cells by blocking PI3K signaling, inhibiting the mammalian target of rapamycin (TOR), upregulating ATG4, and colocalizing with LC3 in autophagosomes (2). DIRAS also interacts with C-RAF and downregulates mitogen-activated protein kinase kinases (MEK) to restrict cell migration (3).

Molecular Weight: Predicted: 25 kDa

Observed: 24 kDa

Gene ID: 9077

NCBI Accession: NP_004666

UniProt: O95661