Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
MYH3 antibody is human, mouse and rat reactive. MYH3 antibody is predicted to not cross-react with other members of the myosin heavy chain family.
Purification
MYH3 antibody is affinity chromatography purified via peptide column.
Immunogen
MYH3 antibody was raised against a 19 amino acid peptide near the amino terminus of human MYH3. The immunogen is located within amino acids 30 - 80 of MYH3.
Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells (1). MYH3 (myosin, heavy chain, skeletal muscle, embryonic) plays a significant role in skeletal muscle development (2) and is also essential for the proper morphology and function of the developing heart (3). Mutations in this gene have been associated with Freeman-Sheldon syndrome and Sheldon-Hall syndrome (4).