MYH8 antibody is human, mouse and rat reactive. MYH8 antibody is predicted to not cross-react with other members of the myosin heavy chain family.
Purification
MYH8 antibody is affinity chromatography purified via peptide column.
Immunogen
MYH8 antibody was raised against a 19 amino acid peptide near the amino terminus of human MYH8. The immunogen is located within amino acids 60 - 110 of MYH8.
MYH8
Reactivity: Human
Host: Rabbit
Polyclonal
Alexa Fluor 750
Application Notes
MYH8 antibody can be used for detection of MYH8 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
MYH8 antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
MYH8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Target
MYH8
(Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8))
Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells (1). MYH8 (myosin, heavy chain 8, skeletal muscle, perinatal) is a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction (2,3). This gene is predominantly expressed in fetal skeletal muscle. MYH8 is regulated by phosphorylation via myosin light chain kinase (MLCK) and by intracellular Ca2+ concentrations (3). A mutation in this gene results in trismus-pseudocamptodactyly syndrome (4).