Deltex Homolog 4 antibody (AA 230-280)

Catalog No. ABIN6992091

Product Details anti-Deltex Homolog 4 Antibody

Target: Deltex Homolog 4 (DTX4) (Deltex Homolog 4 (Drosophila) (DTX4))

Binding Specificity: AA 230-280

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Deltex Homolog 4 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: DTX4 antibody is human and mouse reactive. At least two isoforms of DTX4 are known to exist, this antibody will detect both isoforms. DTX4 antibody is predicted to not cross-react with other DTX protein family members.

Purification: DTX4 antibody is affinity chromatography purified via peptide column.

Immunogen: DTX4 antibody was raised against a 19 amino acid peptide near the center of human DTX4. The immunogen is located within amino acids 230 - 280 of DTX4.

Isotype: IgG

Application Details

Application Notes: DTX4 antibody can be used for detection of DTX4 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: DTX4 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: DTX4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for Deltex Homolog 4

Target: Deltex Homolog 4 (DTX4) (Deltex Homolog 4 (Drosophila) (DTX4))

Alternative Name: DTX4 (DTX4 Products)

Synonyms: RNF155 antibody, AI449438 antibody, BC044798 antibody, D930042H13 antibody, zgc:163052 antibody, LRRGT00037 antibody, deltex E3 ubiquitin ligase 4 antibody, deltex 4, E3 ubiquitin ligase antibody, DTX4 antibody, Dtx4 antibody, dtx4 antibody

Background: The Deltex 4 E3 ubiquitin ligase (DTX4) is a member of a protein family that is involved in Notch signaling and neurogenesis (1). Recent studies have shown that DTX4 is also associated with the innate immune response (2). Following the activation of type I interferon signaling, the Nod-like receptor protein 4 (NLRP4) recruits DTX4 for Lys48-linked polyubiquitination and degradation of the kinase TBK1 (NAK), thereby maintaining immune homeostasis during antiviral innate immunity (2).

Molecular Weight: Predicted: 68 kDa

Observed: 68 kDa

Gene ID: 23220

NCBI Accession: NP_055992

UniProt: Q9Y2E6

Pathways: Notch Signaling