RHOT1 antibody (N-Term)
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- Target See all RHOT1 Antibodies
- RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))
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Binding Specificity
- AA 40-90, N-Term
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RHOT1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- RHOT1 antibody is human, mouse and rat reactive. At least three isoforms of RHOT1 are known to exist, this antibody will detect all three isoforms.
- Purification
- RHOT1 antibody is affinity chromatography purified via peptide column.
- Immunogen
- RHOT1 antibody was raised against a 15 amino acid peptide near the amino terminus of human RHOT1. The immunogen is located within amino acids 40 - 90 of RHOT1.
- Isotype
- IgG
- Top Product
- Discover our top product RHOT1 Primary Antibody
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- Application Notes
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RHOT1 antibody can be used for detection of RHOT1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in rat samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- RHOT1 antibody is supplied in PBS containing 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,4 °C
- Storage Comment
- RHOT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
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- Target
- RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))
- Alternative Name
- RHOT1 (RHOT1 Products)
- Synonyms
- rhot1 antibody, zgc:55581 antibody, zgc:77063 antibody, wu:fd14e11 antibody, wu:fi14a05 antibody, arht2 antibody, miro-2 antibody, rasl antibody, ARHT1 antibody, MIRO-1 antibody, MIRO1 antibody, 2210403N23Rik antibody, AA415293 antibody, AF244542 antibody, AI834919 antibody, Arht1 antibody, C430039G08Rik antibody, Miro1 antibody, miro1 antibody, si:dkeyp-97g3.6 antibody, ras homolog family member T1a antibody, ras homolog family member T2 antibody, ras homolog family member T1 antibody, ras homolog family member T1 L homeolog antibody, rhot1a antibody, rhot2 antibody, RHOT1 antibody, rhot1 antibody, rhot1.L antibody, Rhot1 antibody, rhot1b antibody
- Background
- The Ras homolog family member T1 (RHOT) is an atypical Rho Ca2+-binding GTPase that localizes to the mitochondria (1). RHOT1, the related protein RHOT2, the adaptor protein Milton, and the PTEN induced putative kinase 1 (PINK1), form a complex that is involved in axonal transport of mitochondria (2,3). Both PINK1 and Parkin target RHOT1 for phosphorylation and degradation, causing the arrest of mitochondrial motility (4).
- Molecular Weight
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Predicted: 76 kDa
Observed: 78 kDa - Gene ID
- 55288
- NCBI Accession
- NP_001028740
- UniProt
- Q8IXI2
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