VEZT antibody (AA 480-530)

Catalog No. ABIN6992059

Product Details anti-VEZT Antibody

Target: VEZT (Vezatin, Adherens Junctions Transmembrane Protein (VEZT))

Binding Specificity: AA 480-530

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEZT antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: VEZT antibody is human, mouse and rat reactive. Multiple isoforms are known to exist.

Purification: VEZT antibody is affinity chromatography purified via peptide column.

Immunogen: VEZT antibody was raised against a 17 amino acid peptide near the center of human VEZT. The immunogen is located within amino acids 480 - 530 of VEZT.

Isotype: IgG

Application Details

Application Notes: VEZT antibody can be used for detection of VEZT by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: VEZT antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: VEZT antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for VEZT

Target: VEZT (Vezatin, Adherens Junctions Transmembrane Protein (VEZT))

Alternative Name: VEZT (VEZT Products)

Synonyms: DKFZp469O2222 antibody, VEZATIN antibody, 6330418D12 antibody, AI854408 antibody, vezatin antibody, vezatin, adherens junctions transmembrane protein antibody, vezatin, adherens junctions transmembrane protein L homeolog antibody, VEZT antibody, vezt antibody, Vezt antibody, vezt.L antibody

Background: Vezatin (VEZT) is a transmembrane protein which localizes to adherens junctions and binds to myosin VIIA and the cadherin-catenins complex (1). Expression of this gene in gastric cancer tissues is decreased due to hypermethylation of the promoter. Expression of this gene may also be inhibited by binding of a specific microRNA to a target sequence in the 3' UTR of the transcripts (2). VEZT has also been shown to be required for morphogenesis of the preimplantation mouse embryo (3).

Molecular Weight: Predicted: 63, 68, 78, 81, 83, 86 kDa

Observed: 60 kDa

Gene ID: 55591

NCBI Accession: NP_060069

UniProt: Q9HBM0