Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
SMURF1 antibody is human, mouse and rat reactive. At least three isoforms of SMURF1 are known to exist this antibody will recognize all three isoforms. SMURF1 is predicted to not cross-react with SMURF2.
Purification
SMURF1 antibody is affinity chromatography purified via peptide column.
Immunogen
SMURF1 antibody was raised against a 16 amino acid peptide near the amino terminus of human SMURF1. The immunogen is located within amino acids 180 - 230 of SMURF1.
SMURF1
Reactivity: Human
WB, ELISA
Host: Mouse
Polyclonal
unconjugated
Application Notes
SMURF1 body can be used for detection of SMURF1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
SMURF1 antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
SMURF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Target
SMURF1
(SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))
4930431E10Rik antibody, mKIAA1625 antibody, RGD1309707 antibody, SMAD specific E3 ubiquitin protein ligase 1 antibody, E3 ubiquitin-protein ligase SMURF1 antibody, SMAD specific E3 ubiquitin protein ligase 1 L homeolog antibody, SMURF1 antibody, LOC100540915 antibody, smurf1 antibody, Smurf1 antibody, smurf1.L antibody
Background
SMURF1 (SMAD ubiquitin regulatory factor 1) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2). SMURF1 negatively regulates osteoblast activity and response to bone morphogenesis protein (BMP) through controlling MEKK2 degradation (3). SMURF1 has recently been shown to play a role in breast cancer cell migration and invasion through the downregulation of RhoA (4).