LIN54 antibody (C-Term)

Catalog No. ABIN6992048

Product Details anti-LIN54 Antibody

Target: LIN54 (Lin-54 Homolog (LIN54))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIN54 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: LIN54 antibody is human, mouse, and rat reactive. Multiple isoforms of LIN54 are known to exist.

Purification: LIN54 antibody is affinity chromatography purified via peptide column.

Immunogen: LIN54 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human LIN54. The immunogen is located within the last 50 amino acids of LIN54.

Isotype: IgG

Application Details

Application Notes: LIN54 antibody can be used for detection of LIN54 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: LIN54 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: LIN54 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for LIN54

Target: LIN54 (Lin-54 Homolog (LIN54))

Alternative Name: LIN54 (LIN54 Products)

Synonyms: CXCDC1 antibody, JC8.6 antibody, MIP120 antibody, 4832414L08 antibody, AI461788 antibody, mKIAA2037 antibody, RGD1311361 antibody, lin-54 DREAM MuvB core complex component antibody, lin-54 homolog (C. elegans) antibody, LIN54 antibody, Lin54 antibody

Background: LIN54 is a component of the LIN, or DREAM, complex, an essential regulator of cell cycle genes. This complex functions as a transcriptional repressor by binding to promoters of E2F target genes that regulate the G1/S transition (1). LIN54 is required for cell cycle progression and can bind to the cdc2 promoter in a sequence-specific manner (2).

Molecular Weight: Predicted: 88 kDa

Observed: 82 kDa

Gene ID: 132660

NCBI Accession: NP_919258

UniProt: Q6MZP7

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases