Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
Specificity
OASL antibody is human, mouse and rat reactive.
Purification
OASL antibody is affinity chromatography purified via peptide column.
Immunogen
OASL antibody was raised against a 17 amino acid peptide near the amino terminus of human OASL. The immunogen is located within the first 50 amino acids of OASL.
OASL
Reactivity: Mouse, Rat
IF (cc), IF (p)
Host: Rabbit
Polyclonal
AbBy Fluor® 350
Application Notes
OASL antibody can be used for detection of OASL by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunocytochemistry at 2.5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
OASL Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
OASL antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
OASL (2'-5'-oligoadenylate synthetase-like), also known as p59OASL or TRIP14 (thyroid receptor-interacting protein 14), is a 514 amino acid protein that exists as two alternatively spliced isoforms, designated p56 and p30, and contains two ubiquitin-like domains (1). It is widely expressed in a variety of tissues and interacts with the ligand binding domain of the thyroid receptor (TR) and is able to bind double-stranded RNA and DNA, possibly playing a role in RNA degradation and the overall inhibition of protein synthesis (2-3). Methyl CpG-binding protein 1 (MBD1), which functions as a transcriptional repressor, was identified as a strong p59 OASL interactor (4).