USP25 antibody (AA 470-520)

Catalog No. ABIN6991995

Product Details anti-USP25 Antibody

Target: USP25 (Ubiquitin Specific Peptidase 25 (USP25))

Binding Specificity: AA 470-520

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: USP25 antibody is human and mouse reactive. Multiple isoforms of USP25 are known to exist.

Purification: USP25 antibody is affinity chromatography purified via peptide column.

Immunogen: USP25 antibody was raised against a 17 amino acid peptide near the center of human USP25. The immunogen is located within amino acids 470 - 520 of USP25.

Isotype: IgG

Application Details

Application Notes: USP25 antibody can be used for detection of USP25 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: USP25 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: USP25 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for USP25

Target: USP25 (Ubiquitin Specific Peptidase 25 (USP25))

Alternative Name: USP25 (USP25 Products)

Synonyms: USP25 antibody, zgc:55791 antibody, wu:fi33c05 antibody, wu:fo67e06 antibody, wu:fp97a04 antibody, usp25 antibody, MGC81666 antibody, usp21 antibody, USP21 antibody, ubiquitin specific peptidase 25 antibody, universal stress protein UspA antibody, ubiquitin specific peptidase 25 L homeolog antibody, USP25 antibody, RR_RS03930 antibody, usp25 antibody, usp25.L antibody, Usp25 antibody

Background: USP25 (ubiquitin specific peptidase 25), also known as USP21, belongs to the peptidase C19 family and is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response (1). It contains one UBA-like domain and two UIM (ubiquitin-interacting motif) repeats. Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle-specific isoform (2). The long isoform of USP25 (USP25m) is upregulated in myogenesis and is implicated in regulation of muscular differentiation and function. USP25 is a deubiquitinating enzyme (DUB) that negatively regulates IL-17-triggered signaling (3,4).

Molecular Weight: Predicted: 116 kDa

Observed: 105 kDa

Gene ID: 29761

NCBI Accession: NP_037528

UniProt: Q9UHP3