PHC3 antibody (N-Term)

Catalog No. ABIN6991943

Product Details anti-PHC3 Antibody

Target: PHC3 (Polyhomeotic Homolog 3 (PHC3))

Binding Specificity: AA 240-290, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: PHC3 antibody is human, mouse and rat specific. At least six isoforms of PHC3 are known to exist.

Purification: PHC3 antibody is affinity chromatography purified via peptide column.

Immunogen: PHC3 antibody was raised against a 19 amino acid peptide near the amino terminus of human PHC3. The immunogen is located within amino acids 240 - 290 of PHC3.

Isotype: IgG

Application Details

Application Notes: PHC3 antibody can be used for detection of PHC3 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PHC3 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PHC3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for PHC3

Target: PHC3 (Polyhomeotic Homolog 3 (PHC3))

Alternative Name: PHC3 (PHC3 Products)

Synonyms: EDR3 antibody, HPH3 antibody, E030046K01Rik antibody, Edr3 antibody, Hph3 antibody, polyhomeotic homolog 3 antibody, polyhomeotic-like 3 (Drosophila) antibody, Phc3 antibody, PHC3 antibody

Background: Polycomb group (PcG) proteins assemble into multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes throughout development (1). PHC3 (Polyhomeotic-like protein 3), also known as Early development regulatory protein 3, is a 983 amino acid nuclear protein that is a component of the PcG multiprotein PRC1 complex (2). PRC1 complex acts via chromatin remodeling and modification of histones. PHC3 contains 1 FCS-type zinc finger and SAM (sterile alpha motif) domain (2-3). PHC 3 has been regarded as a candidate tumor suppressor of osteosarcoma (4).

Molecular Weight: Predicted: 109 kDa

Observed: 120 kDa

Gene ID: 80012

NCBI Accession: NP_079223

UniProt: Q8NDX5