BHLHA15 antibody (AA 60-110)

Catalog No. ABIN6991922

Product Details anti-BHLHA15 Antibody

Target: BHLHA15 (Basic Helix-Loop-Helix Family, Member A15 (BHLHA15))

Binding Specificity: AA 60-110

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BHLHA15 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: BHLHA15 antibody is human, mouse and rat reactive.

Purification: BHLHA15 antibody is affinity chromatography purified via peptide column.

Immunogen: BHLHA15 antibody was raised against a 18 amino acid peptide near the center of human BHLHA15. The immunogen is located within amino acids 60 - 110 of BHLHA15.

Isotype: IgG

Application Details

Application Notes: BHLHA15 antibody can be used for detection of BHLHA15 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: BHLHA15 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: BHLHA15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for BHLHA15

Target: BHLHA15 (Basic Helix-Loop-Helix Family, Member A15 (BHLHA15))

Alternative Name: BHLHA15 (BHLHA15 Products)

Synonyms: BHLHB8 antibody, MIST1 antibody, 1810009C13Rik antibody, Bhlhb8 antibody, MIST-1 antibody, Mist1 antibody, bHlH antibody, basic helix-loop-helix family member a15 antibody, basic helix-loop-helix family, member a15 antibody, BHLHA15 antibody, Bhlha15 antibody

Background: BHLHA15 (basic helix-loop-helix protein 15), also known as MIST1 (muscle intestine and stomach expression 1) belongs to the bHLH family of transcription factors and plays a role in regulating the transcriptional activity of MYOD1 in muscle cell development as well as serving as a key regulator of acinar cell function (1,2). BHLHA15 contains a basic helix-loop-helix (bHLH) domain and is capable of binding to E-box motifs as a homodimer or a heterodimer with E-proteins. It may also negatively regulate bHLH-mediated transcription through a N-terminal repressor domain (3). It is expressed in mammary epithelial cells and is essential for the regulation of mammary gland development (4).

Molecular Weight: Predicted: 21 kDa

Observed: 24 kDa

Gene ID: 168620

NCBI Accession: NP_803238

UniProt: Q7RTS1