FIL1h antibody (C-Term)

Catalog No. ABIN6991916

Product Details anti-FIL1h Antibody

Target: FIL1h (Interleukin 1 eta (FIL1h))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FIL1h antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: IL-36B antibody is human specific. At least two isoforms of IL-36B are known to exist, this antibody will only detect the longer isoform. IL-36B antibody will not cross-react with IL-36A or IL-36G.

Purification: IL-36B antibody is affinity chromatography purified via peptide column.

Immunogen: IL-36B antibody was raised against a 16 amino acid peptide near the carboxy terminus of human IL-36B. The immunogen is located within the last 50 amino acids of IL-36B.

Isotype: IgG

Application Details

Application Notes: IL-36B antibody can be used for detection of IL-36B by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: IL-36B antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: IL-36B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for FIL1h

Target: FIL1h (Interleukin 1 eta (FIL1h))

Alternative Name: IL-36B (FIL1h Products)

Synonyms: 2310043N20Rik antibody, Il36b antibody, FIL1 antibody, FIL1-(ETA) antibody, FIL1H antibody, FILI-(ETA) antibody, IL-1F8 antibody, IL-1H2 antibody, IL1-ETA antibody, IL1F8 antibody, IL1H2 antibody, Il1f8 antibody, interleukin 1 family, member 8 antibody, interleukin 36 beta antibody, interleukin 36, beta antibody, Il1f8 antibody, IL36B antibody, Il36b antibody

Background: IL-36B is is a member of the interleukin 1 cytokine family whose gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2 (1). IL-36B is thought to activate the NF-kappaB pathway through IL-1 receptor family members IL-1RL2 and IL-1RAcP (2). Like the related proteins IL-36A and IL-36G, IL-36B requires post-translational processing for full agonist activity, but the cleavage mechanism is currently unknown (3). The IL-36 cytokines have been suggested to amplify Th1 responses by enhancing proliferation and Th1 polarization of naive CD4+ T cells (4).

Molecular Weight: Predicted: 18 kDa

Observed: 17 kDa

Gene ID: 27177

NCBI Accession: NP_055253

UniProt: Q9NZH7