WAC antibody (AA 210-260)

Catalog No. ABIN6991845

Product Details anti-WAC Antibody

Target: WAC (WW Domain Containing Adaptor with Coiled-Coil (WAC))

Binding Specificity: AA 210-260

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WAC antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: At least two isoforms of WAC are known to exist, this antibody will detect the larger isoform.

Purification: WAC Antibody is affinity chromatography purified via peptide column.

Immunogen: WAC antibody was raised against a 19 amino acid peptide near the center of human WAC . The immunogen is located within amino acids 210 - 260 of WAC.

Isotype: IgG

Application Details

Application Notes: WAC Antibody can be used for detection of WAC by Western blot at 1 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: WAC Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: WAC antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for WAC

Target: WAC (WW Domain Containing Adaptor with Coiled-Coil (WAC))

Alternative Name: WAC (WAC Products)

Synonyms: BM-016 antibody, PRO1741 antibody, Wwp4 antibody, 1110067P07Rik antibody, A230035H12Rik antibody, AI256735 antibody, AI256776 antibody, DKFZp468H162 antibody, wac antibody, wu:fc13f04 antibody, wu:fj68f01 antibody, zgc:55755 antibody, RGD1562407 antibody, zgc:101828 antibody, WW domain containing adaptor with coiled-coil antibody, WW domain containing adaptor with coiled-coil a antibody, WW domain containing adaptor with coiled-coil b antibody, WAC antibody, Wac antibody, wac antibody, waca antibody, wacb antibody

Background: WAC Antibody: The WW domain containing adaptor with coiled-coil protein (WAC) contains a WW domain that mediates protein-protein interactions and colocalizes with RNA splicing factor SC35. Further studies have indicated that WAC is a functional partner of the RNF20/40 complex that ubiquitinates Histone H2B, and that WAC regulates H2B ubiquitination. WAC targets RNF20/40 to associate with RNA polymerase II complex for H2B ubiquitination at active transcription sites. WAC-dependent transcription is also important for cell-cycle checkpoint activation in response to genotoxic stress.

Molecular Weight: Predicted: 71 kDa

Observed: 75 kDa

Gene ID: 51322

NCBI Accession: NP_057712

UniProt: Q9BTA9

Pathways: Chromatin Binding