Western Blotting (WB), ELISA, Immunofluorescence (IF)
Specificity
NGN2 antibody is predicted to not cross-react with neurogenin 1.
Purification
NGN2 Antibody is affinity chromatography purified via peptide column.
Immunogen
Rabbit polyclonal NGN2 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human NGN2. The immunogen is located within the last 50 amino acids of NGN2.
NEUROG2
Reactivity: Human
WB, ELISA
Host: Mouse
Monoclonal
3D8
unconjugated
Application Notes
NGN2 antibody can be used for detection of NGN2 by Western blot at 1 - 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
NGN2 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
NGN2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
NGN2 Antibody: Neurogenin-2 (NGN2) is a neural-specific basic helix-loop-helix (bHLH) transcription factor that can specify a neuronal fate on ectodermal cells and is expressed in neural progenitor cells within the developing central and peripheral nervous systems. NGN2 is thought to work with Nurr1 to play a role in the differentiation and survival of midbrain dopaminergic neurons. It has also been suggested for use in human embryonic neural progenitors as a graft for spinal cord injuries