Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
Multiple isoforms of VNN3 are known to exist.
Purification
VNN3 Antibody is affinity chromatography purified via peptide column.
Immunogen
VNN3 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human VNN3. The immunogen is located within the last 50 amino acids of VNN3.
VNN3
Reactivity: Human
WB, ELISA, IHC
Host: Rabbit
Polyclonal
Biotin
Application Notes
VNN3 antibody can be used for detection of VNN3 by Western blot at 0.5 and 1 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
VNN3 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
VNN3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
VNN3 Antibody: The vanin family is a novel group of ectoenzymes that function in tissue repair and plays a role in oxidative-stress response. As both secreted and membrane proteins, the vanin family members have been implicated as therapeutic targets in inflammatory disease. VNN3 (vascular non-inflammatory molecule 3), also known as Vanin3, is a 501 amino acid GPI-anchored amidohydrolase that is widely expressed and is found at highest levels in blood and liver. Induced by Th17 / Th1 type cytokines, VNN3 converts pantetheine into pantothenic acid. Containing one CN hydrolase domain, VNN3 is encoded by a gene that maps to human chromosome 6q23.2.