Western Blotting (WB), ELISA, Immunofluorescence (IF)
Specificity
Multiple isoforms of DCP2 are known to exist.
Purification
DCP2 Antibody is affinity chromatography purified via peptide column.
Immunogen
DCP2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DCP2. The immunogen is located within amino acids 340 - 390 of DCP2.
DCP2
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
DCP2 antibody can be used for detection of DCP2 by Western blot at 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
DCP2 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
DCP2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
DCP2 Antibody: The removal, or decapping, of eukaryotic mRNA is an important step in the degradation of mRNA. Decapping protein 2 (DCP2) is the major mRNA decapping enzyme in cells. It is a member of the Nudix hydrolases superfamily of proteins that predominantly catalyze the hydrolysis of small nucleoside diphosphate substrates linked to another moiety. DCP2 is widely expressed in multiple tissues at varying levels, with highest expression seen in testis and brain.