Western Blotting (WB), ELISA, Immunofluorescence (IF)
Specificity
Multiple isoforms of SGSM2 are known to exist. SGSM2 antibody is predicted to not cross react with other SGSM family proteins.
Purification
SGSM2 Antibody is affinity chromatography purified via peptide column.
Immunogen
SGSM2 antibody was raised against an 18 amino acid synthetic peptide near the center of human SGSM2. The immunogen is located within amino acids 650 - 700 of SGSM2.
SGSM2
Reactivity: Mouse, Human, Rat
WB, EIA, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
SGSM2 antibody can be used for detection of SGSM2 by Western blot at 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
SGSM2 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
SGSM2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
SGSM2
(Small G Protein Signaling Modulator 2 (SGSM2))
RUTBC1 antibody, D630003G22Rik antibody, R74628 antibody, Rutbc1 antibody, mKIAA0397 antibody, small G protein signaling modulator 2 antibody, SGSM2 antibody, Sgsm2 antibody
Background
SGSM2 Antibody: Small G proteins such as RAP and RAB proteins are the key molecules in intracellular signal transduction and vesicle transportation. A novel protein family small G protein signaling modulator (SGSM) consisting of three members SGSM1-3 bind to RAP and RAB family proteins. All three SGSM proteins possess both a RUN domain and a TBC domain. SGSM2 is a Rab9A effector that activates GTP hydrolysis by Rab32 and Rab33B proteins.