Western Blotting (WB), Immunofluorescence (IF), ELISA
Specificity
At least three isoforms are known to exist, this antibody will detect the two largest isoforms. TCTEX1D1 is predicted to not cross-react with other members of the TCTEX-type family.
Purification
TCTEX1D1 Antibody is affinity chromatography purified via peptide column.
Immunogen
TCTEX1D1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human. The immunogen is located within amino acids 30 - 80 of TCTEX1D1.
TCTEX1D1
Reactivity: Human
WB, IF, EIA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
TCTEX1D1 antibody can be used for detection of TCTEX1D1 by Western blot at 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
TCTEX1D1 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
TCTEX1D1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
TCTEX1D1 Antibody: Dyneins are multi-subunit, high molecular weight ATPases that interact with microtubules to generate force by converting the chemical energy of ATP into the mechanical energy of movement. Cytoplasmic or axonemal Dynein is an approximately 12 subunit complex of two heavy chains, two intermediate chains to anchor Dynein to its cargo, four smaller intermediate chains and several light chains. It performs functions necessary for cell survival such as organelle transport and centrosome assembly. TCTEX1 is a cytoplasmic dynein light chain found in a complex with Na+ CP type X (SCN10A). TCTEX1D1, also called TCTEX1 domain containing 1, belongs to the dynein light chain TCTEX-type family. Its function is still under investigation.