DDX60 antibody (AA 1140-1190)

Catalog No. ABIN6991720

Product Details anti-DDX60 Antibody

Target: DDX60 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 60 (DDX60))

Binding Specificity: AA 1140-1190

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX60 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Specificity: At least three isoforms of DDX60 are known to exist, this antibody will detect all three isoforms.

Purification: DDX60 Antibody is affinity chromatography purified via peptide column.

Immunogen: DDX60 antibody was raised against a 17 amino acid synthetic peptide near the center of human DDX60. The immunogen is located within amino acids 1140 - 1190 of DDX60.

Isotype: IgG

Application Details

Application Notes: DDX60 antibody can be used for detection of DDX60 by Western blot at 1 - 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: DDX60 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: DDX60 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for DDX60

Target: DDX60 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 60 (DDX60))

Alternative Name: DDX60 (DDX60 Products)

Synonyms: 9830118M07 antibody, RGD1560014 antibody, DExD/H-box helicase 60 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 60 antibody, DEXD/H-box helicase 60 antibody, DDX60 antibody, Ddx60 antibody

Background: DDX60 Antibody: DDX60 is a DEXD/H Box RNA helicase and the human homolog of the S. cerevisiae Ski2, a cofactor of RNA exosome. Expression of DDX60 increases after viral infection and binds to endogenous RIG-I, a cytoplasmic viral RNA sensor, as well as other RIG-I-like receptors such as MDA5 and LGP2. These proteins activate TBK1 and IKK-e, signaling IRF3 and NF-kB-responsive genes in the innate immune response pathway. DDX60 is required for the RIG-I- or MDA5-dependent response to viral infection, but is dispensable for TLR3-mediated signaling.

Molecular Weight: Predicted: 125, 185, 188 kDa

Observed: 190 kDa

Gene ID: 55601

NCBI Accession: NP_060101

UniProt: Q8IY21